PARAMETER ANALYSIS OF CLUSTERS OF MELTING TEMPERATURES OF DNA CHAINS
Cysewska-Sobusiak Anna, Lerczak Agata, Kasprzak Marta, Markiewicz Wojciech T.
DNA sequencing, clustering, hybridization experiment.
In the paper the problem of DNA sequencing by hybridization (SBH) is considered.With the developed software, MELTEM, several assembling procedures are used to ease acollecting a subset of oligonucleotides that would melt under practically identical conditionsin a hybridization experiment. Some clustering approaches implemented in the program arecompared. The algorithms of MELTEM are presented as well as the selected examples of amelting temperature analysis.